2024 principles of fluorescence techniques

apr 15 - 18, 2024 | urbana-champaign, il

Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques workshop will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation.

The workshop is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FFS applications.

Participants are recommended to have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending. Interactions between participants and lecturers will be fostered. Students will have ample opportunity to personally explain their research programs and ask questions about the applicability of specific fluorescence techniques to their workshop lecturers. Topics addressed in this workshop include:

  • Basic Definitions and Principles of Fluorescence
  • Steady-state Fluorescence (spectra, kinetics, polarization)
  • Time-resolved Fluorescence (lifetime, anisotropy, time-resolved spectra)
  • Fluorescence Probes
  • Instrumentation
  • Data Manipulation and Data Analysis
  • Confocal and Multiphoton Fluorescence Microscopy
  • FFS, Fluorescence Fluctuation Spectroscopy
  • FLIM, Fluorescence Lifetime Imaging
  • Particle Tracking
  • Single Molecule Imaging
  • Super-resolution techniques, STED

The number of participants to the course is limited:

Lectures: Limited to a total of 70 participants (auditorium size).

Practicals: Limited to a total of 30 participants (interactive, small-group sessions).

Participation is granted on a first-registered, first-served basis.

Sponsored by:

Tentative Agenda

April 4

1:30 PM - 2:00 PM
Registration & Introductions
2:00 PM - 2:30 PM
Welcome Address
Prof. Stephen A. Boppart
2:30 PM - 3:00 PM
Short History of Fluorescence
Dr. Beniamino Barbieri
3:00 PM - 4:30 PM
Basic Fluorescence Principles I
Overview, Excitation and Emission Spectra, Quantum Yields, Polarization
Prof. David M. Jameson
4:30 PM - 5:00 PM
Coffee Break
5:00 PM - 6:30 PM
Basic Fluorescence Principles II
Time-Resolved Fluorescence and Quenching
Prof. David M. Jameson
6:30 PM - 12:00 AM
Networking Mixer

April 5

8:00 AM - 8:30 AM
Breakfast
8:30 AM - 10:00 AM
Basic Fluorescence Principles III
FRET: In Vitro and In Vivo Fluorescence Probes
Prof. David M. Jameson
10:00 AM - 10:30 AM
Coffee Break
10:30 AM - 11:00 AM
Basic Instrumentation
Prof. David M. Jameson
11:00 AM - 12:30 PM
Fluorescence Microscopy
Dr. Beniamino Barbieri
12:30 PM - 1:30 PM
Lunch
1:30 PM - 2:00 PM
Group Picture and Experiment Group Assignment
2:00 PM - 4:00 PM
Laboratory Practicals
4:00 PM - 4:15 PM
Coffee Break
4:15 PM - 6:00 PM
Laboratory Practicals

April 6

8:00 AM - 8:30 AM
Breakfast
8:30 AM - 10:00 AM
FCS, Autocorrelation; PCH, Cross-correlation
Prof. Silviya Zustiak
10:00 AM - 10:30 AM
Coffee Break
10:30 AM - 12:00 PM
RICS, N&B, and FLIM
Prof. Parijat Sengupta
12:00 PM - 1:00 PM
Lunch
1:00 PM - 2:30 PM
Monitoring Dynamic Protein Interactions in the Living Cell Nucleus
Prof. Richard Day
2:30 PM - 4:30 PM
Laboratory Practicals
4:30 PM - 4:45 PM
Coffee Break
4:45 PM - 6:00 PM
Laboratory Practicals

April 7

8:00 AM - 8:30 AM
Breakfast
8:30 AM - 9:30 AM
Label-free Multimodal Molecular Imaging of Breast Cancer Development
Prof. Stephen A. Boppart
9:30 AM - 10:30 AM
From Photons to Function: Quantitative Mining of Number and Brightness
Prof. Catherine Royer
10:30 AM - 10:45 AM
Coffee Break
10:45 AM - 11:45 AM
Fluorescence Detection of Pressure-Dependent Protein Folding in vitro and in Cells
Prof. Martin Gruebele
11:45 AM - 12:00 PM
Closing Remarks/Award of Participation Diplomas

Instructors

Dr. Beniamino Barbieri

Dr. Beniamino Barbieri

ISS, Inc. | President | 1602 Newton Dr. | Champaign, IL 61822 | Tel: 217-359-8681

Prof. Stephen A. Boppart

Prof. Stephen A. Boppart

Beckman Institute for Advanced Science and Technology | University of Illinois at Urbana-Champaign | 405 North Mathews Avenue | Urbana, IL 61801 | Tel: (217) 333-8598

Prof. Richard Day

Prof. Richard Day

Department of Cellular & Integrative Physiology | Indiana University School of Medicine | 635 Barnhill Drive, Room 333 | Indianapolis, IN 46202-5120 | USA

Prof. Martin Gruebele

Prof. Martin Gruebele

James R. Eiszner Chair in Chemistry, | Professor of Physics, and Professor of Biophysics and Computational Biology

Prof. David M. Jameson

Prof. David M. Jameson

University of Hawaii at Manoa | Department of Cell and Molecular Biology | John A. Burns School of Medicine | Honolulu, HI 96822 | USA | Tel: 808-956-5034 | Website

Prof. Catherine Royer

Prof. Catherine Royer

CNRS | Centre de Biochimie Structurale | 29, route de Navacelles | 34090 Montpellier CEDEX | France | Tel: +33-04 67 41 79 02

Prof. Parijat Sengupta

Prof. Parijat Sengupta

Department of Bioengineering and Beckman Institute | University of Illinois at Urbana-Champaign | Website

Prof. Silviya Zustiak

Prof. Silviya Zustiak

Assistant Professor, Biomedical Engineering, SLU | Adjunct Assistant Professor, Chemical and Biochemical Engineering, MST | Parks College of Engineering, Aviation and Technology | 3507 Lindell Blvd, St Louis, MO 63103 | phone: 314-977-8331

Travel

Location

The Principles of Fluorescence Techniques workshop will be held at:

Beckman Institute for Advanced Science and Technology
The University of Illinois
405 North Mathews Avenue
Urbana, IL 61801

Airports

The airport code for Champaign is CMI. Other close airports are Chicago Midway (MDW), Chicago O'Hare (ORD), Indianapolis (IND) and Bloomington-Normal (BMI).
Directions from CMI
Directions from BMI
Directions from IND
Directions from ORD
Directions from MDW

Trains

Amtrak offers a line from Chicago (CHI) to Champaign (CHM).

Bus/Shuttle

There is a shuttle service from Chicago to Champaign with Peoria Charter Coach Company.

Hotel

Hampton Inn
1200 W University Ave
Urbana, IL 61801
Phone: 217-337-1100
Click on the Hampton Inn link above to reserve your room with the Fluorescence Foundation block, called Fluorescence.

Illini Union Hotel
1401 W Green St
Urbana, IL 61801
Phone: 217-333-1241

Parking

University parking passes are available for $13/day. These are good at any university metered parking space. If you would like to purchase these passes please contact the workshop coordinator for assistance, and we will have it available at registration for you. There are metered parking spaces nearby on Wright street, the Beckman circle drive and the campus parking garage east of the Beckman Institute.

Register

Registration is closed for this event.