2016 advanced applications of fluorescence techniques

jul 4 - 6, 2016 | avellino, italy

Fluorescence techniques are being used and applied increasingly in academics and industry. The Advanced Applications of Fluorescence Techniques course will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation.

The course is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications provided by leading companies.

Participants are recommended to have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending. Interactions between participants and lecturers will be fostered. Students will have ample opportunity to personally explain their research programs and ask questions about the applicability of specific fluorescence techniques to their course lecturers. Topics addressed in this course include:

  • Basic Definitions and Principles of Fluorescence
  • Fluorescence Polarization
  • Time-resolved Fluorescence
  • Instrumentation
  • Data Manipulation and Data Analysis
  • Fluorescence Probes
  • Confocal and Multiphoton Fluorescence Microscopy
  • FFS, Fluorescence Fluctuation Spectroscopy
  • FLIM, Fluorescence Lifetime Imaging
  • Single Molecule Imaging

The number of participants to the course is limited:

Lectures: Limited to a total of 70 participants (auditorium size).

Practicals: Limited to a total of 30 participants (interactive, small-group sessions).

Participation is granted on a first-registered, first-served basis.

We encourage you to register as soon as possible.

Sponsored by:

Tentative Agenda

April 15

1:30 PM - 2:00 PM
Registration & Introductions
2:00 PM - 2:30 PM
Welcome to the Beckman Institute
2:30 PM - 3:00 PM
Short History of Fluorescence
Dr. Beniamino Barbieri
3:00 PM - 4:30 PM
Basic Fluorescence Principles I
Overview, Excitation and Emission Spectra,
Quantum Yields, Polarization
Prof. David M. Jameson
4:30 PM - 5:00 PM
Coffee Break
5:00 PM - 6:30 PM
Basic Fluorescence Principles II
Time-Resolved Fluorescence and Quenching
Prof. David M. Jameson
6:30 PM - 12:00 AM
Networking Mixer

April 16

8:00 AM - 8:30 AM
Breakfast
8:30 AM - 10:00 AM
Basic Fluorescence Principles III
FRET: In vitro and In vivo Fluorescence Probes
Prof. David M. Jameson
10:00 AM - 10:30 AM
Coffee Break
10:30 AM - 11:00 AM
Basic Instrumentation
Prof. David M. Jameson
11:00 AM - 12:30 PM
FCS, Autocorrelation; PCH, Cross-correlation
Prof. Michelle Digman
12:30 PM - 1:30 PM
Lunch
1:30 PM - 2:00 PM
Group Picture and Experiment Group Assignment
2:00 PM - 4:00 PM
Laboratory Practicals
4:00 PM - 4:30 PM
Coffee Break
4:30 PM - 6:00 PM
Laboratory Practicals

April 17

8:00 AM - 8:30 AM
Breakfast
8:30 AM - 10:00 AM
RICS, N&B, Scanning FCS
Prof. Michelle Digman
10:00 AM - 10:30 AM
Coffee Break
10:30 AM - 12:00 PM
FLIM, Spectral FLIM, Phasors
Prof. Michelle Digman
12:00 PM - 1:00 PM
Lunch
1:00 PM - 3:00 PM
Laboratory Practicals
3:00 PM - 3:30 PM
Coffee Break
3:30 PM - 5:30 PM
Laboratory Practicals

April 18

8:00 AM - 8:30 AM
Breakfast
8:30 AM - 10:30 AM
Group Discussion
10:30 AM - 11:00 AM
Coffee Break
11:00 AM - 12:00 PM
Free Time
12:00 PM - 1:00 PM
Lunch
1:00 PM - 2:00 PM
Investigating the Voltage Sensor Conformations of Ion Channels using FLIM-FRET
Prof. Gucan 'Gabriel' Dai
2:00 PM - 3:00 PM
Exploiting Biological Autofluorescence
for Label-Free Multimodal Multiphoton Imaging
Prof. Stephen A. Boppart
3:00 PM - 3:30 PM
Closing Remarks/Award of Participation Diplomas

Instructors

Dr. Beniamino Barbieri

Dr. Beniamino Barbieri

ISS, Inc. | President | 1602 Newton Dr. | Champaign, IL 61822 | Tel: 217-359-8681

Prof. Stephen A. Boppart

Prof. Stephen A. Boppart

Beckman Institute for Advanced Science and Technology | University of Illinois at Urbana-Champaign | 405 North Mathews Avenue | Urbana, IL 61801 | Tel: (217) 333-8598

 

Prof. Gucan 'Gabriel' Dai

Department of Biochemistry and Molecular Biology Edward A. Doisy Research Center Saint Louis University School of Medicine 1100 South Grand Blvd. St. Louis, MO 63104

 

Prof. Michelle Digman

Associate Professor Biomedical Engineering 3103 Natural Sciences II Samueli School of Engineering University of California, Irvine Irvine, CA 92697

Prof. David M. Jameson

Prof. David M. Jameson

University of Hawaii at Manoa | Department of Cell and Molecular Biology | John A. Burns School of Medicine | Honolulu, HI 96822 | USA | Tel: 808-956-5034 | Website

Travel

Location

The Principles of Fluorescence Techniques course will be held at:
Istituto di Scienze dell’Alimentazione
Consiglio Nazionale delle Ricerche
83100 Avellino
Italy

Airports

Aereoporto Internazionale di Napoli

Buses (from Napoli to Avellino)

A.I.R. Autoservizi Irpini
SITA Sud S.r.l.

Trains

Trenitalia
Italotreno

Hotel

Hotel De La Ville
Via Palatucci
Avellino

Viva Hotel
Via Circumvallazione 123
Avellino

B&B L'Angoletto
Via Derna 13
Avellino

B&B L'Angoletto Young
Via Guido Dorso 15
Avellino

Register

Registration is closed for this event.