2012 principles of fluorescence techniques

jun 4 - 6, 2012 | urbana-champaign, il

Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques workshop will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation.

The workshop is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FFS applications.

Participants are recommended to have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending. Interactions between participants and lecturers will be fostered. Students will have ample opportunity to personally explain their research programs and ask questions about the applicability of specific fluorescence techniques to their workshop lecturers. Topics addressed in this workshop include:

  • Basic Definitions and Principles of Fluorescence
  • Steady-state Fluorescence (spectra, kinetics, polarization)
  • Time-resolved Fluorescence (lifetime, anisotropy, time-resolved spectra)
  • Fluorescence Probes
  • Instrumentation
  • Data Manipulation and Data Analysis
  • Confocal and Multiphoton Fluorescence Microscopy
  • FFS, Fluorescence Fluctuation Spectroscopy
  • FLIM, Fluorescence Lifetime Imaging
  • Particle Tracking
  • Single Molecule Imaging
  • Super-resolution techniques, STED

The number of participants to the course is limited:

Lectures: Limited to a total of 70 participants (auditorium size).

Practicals: Limited to a total of 30 participants (interactive, small-group sessions).

Participation is granted on a first-registered, first-served basis.

Sponsored by:

Tentative Agenda

May 31

9:00 AM - 9:30 AM
arrival and registration - by Anna Hellriegel
10:00 AM - 10:30 AM
a short history of fluorescence - lecture 1
Dr. Beniamino Barbieri
10:30 AM - 12:00 PM
basic fluorescence principles I - lecture 2
Prof. David M. Jameson
12:00 PM - 1:30 PM
lunch
1:30 PM - 3:00 PM
basic fluorescence principles II - lecture 3
Prof. David M. Jameson
3:00 PM - 4:00 PM
basic instrumentation
Prof. Dr. Martin vandeVen
4:00 PM - 5:00 PM
coffee break, poster session, and discussion
5:00 PM - 7:00 PM
fluorescence microscopy - lecture 4
Prof. Enrico Gratton
8:30 PM - 12:00 AM
dinner reception

June 1

9:00 AM - 10:30 AM
fluorescence probes and labels for biomedical applications I - lecture 5
Dr. Susana Sánchez Donoso
10:30 AM - 11:00 AM
coffee break
11:00 AM - 12:30 PM
fluorescence fluctuation microscopy I (FCS, FCCS, PCH) - lecture 6
Prof. Enrico Gratton
12:30 PM - 2:00 PM
lunch
2:00 PM - 3:00 PM
fluorescence fluctuation microscopy II (N&B) - lecture 7
Dr. Christian Hellriegel
3:00 PM - 4:00 PM
application notes on fluorescence fluctuation microscopy: practical examples of FCS, PCH, N&B - notes 1
Dr. Valeria Caiolfa
4:00 PM - 5:30 PM
coffee break, poster session & group picture
5:30 PM - 7:00 PM
raster image correlation spectroscopy (RICS) and fluorescence lifetime imaging (FLIM) - lecture 8
Prof. Enrico Gratton

June 2

8:30 AM - 1:00 PM
labs: hands-on experiments - session 1
1:00 PM - 2:30 PM
lunch
2:30 PM - 3:30 PM
phasor-FLIM - lecture 9
Prof. Enrico Gratton
3:30 PM - 4:30 PM
application notes on phasor-FLIM: practical examples - notes 2
Dr. Moreno Zamai
4:30 PM - 5:30 PM
closing seminar - laurdan generalized polarization: from cuvette to microscope for the study of cell membrane properties (practical examples)
Dr. Susana Sánchez Donoso
5:30 PM - 6:00 PM
coffee break
6:00 PM - 7:30 PM
open discussion time, closing remarks and award of participation diplomas

June 3

8:00 AM - 1:30 PM
labs: hands-on experiments - session 2
1:30 PM - 3:00 PM
lunch
3:00 PM - 8:00 PM
labs: hands-on experiments - session 3

June 4

8:30 AM - 1:00 PM
labs: hands-on experiments - session 4
1:00 PM - 2:00 PM
lunch

Instructors

Dr. Beniamino Barbieri

Dr. Beniamino Barbieri

ISS, Inc. | President | 1602 Newton Dr. | Champaign, IL 61822 | Tel: 217-359-8681

Dr. Valeria Caiolfa

Dr. Valeria Caiolfa

CNIC | Microscopy and Dynamic Imaging | Melchor Fernández Almagro, 3 | E-28029 Madrid | Spain | Tel: +34 (91) 4531323/9; ext:2206-2207

Dr. Susana Sánchez Donoso

Dr. Susana Sánchez Donoso

Facultad de Ciencias Químicas | Departmento de Polimeros | Universidad de Concepción | Concepción, Chile

Prof. Enrico Gratton

Prof. Enrico Gratton

University of California, Irvine | Department of Biomedical Engineering | Irvine, CA 92697-2715 | USA | Tel: 949-824-2674

Dr. Christian Hellriegel

Dr. Christian Hellriegel

CNIC | Melchor Fernández Almagro, 3 | E-28029 Madrid | Spain

Prof. David M. Jameson

Prof. David M. Jameson

University of Hawaii at Manoa | Department of Cell and Molecular Biology | John A. Burns School of Medicine | Honolulu, HI 96822 | USA | Tel: 808-956-5034 | Website

Dr. Moreno Zamai

Dr. Moreno Zamai

CNIC | Microscopy and Dynamic Imaging | Melchor Fernández Almagro, 3 | E-28029 Madrid | Spain | Tel: +34 (91) 4531323/9; ext:2206-2207

Prof. Dr. Martin vandeVen

Prof. Dr. Martin vandeVen

Hasselt University and School of Life Sciences | Transnationale Universiteit Limburg | Biomedical Research Institute | Agoralaan, Bldg. C., | B-3590-Diepenbeek | Belgium | Tel: +31-(0)11-269233/martin.vandeven@uhasselt.be

Travel

Location

The Principles of Fluorescence Techniques workshop will be held at:

Beckman Institute for Advanced Science and Technology
The University of Illinois
405 North Mathews Avenue
Urbana, IL 61801

Airports

The airport code for Champaign is CMI. Other close airports are Chicago Midway (MDW), Chicago O'Hare (ORD), Indianapolis (IND) and Bloomington-Normal (BMI).
Directions from CMI
Directions from BMI
Directions from IND
Directions from ORD
Directions from MDW

Trains

Amtrak offers a line from Chicago (CHI) to Champaign (CHM).

Bus/Shuttle

There is a shuttle service from Chicago to Champaign with Peoria Charter Coach Company.

Hotel

Hampton Inn
1200 W University Ave
Urbana, IL 61801
Phone: 217-337-1100
Click on the Hampton Inn link above to reserve your room with the Fluorescence Foundation block, called Fluorescence.

Illini Union Hotel
1401 W Green St
Urbana, IL 61801
Phone: 217-333-1241

Parking

University parking passes are available for $13/day. These are good at any university metered parking space. If you would like to purchase these passes please contact the workshop coordinator for assistance, and we will have it available at registration for you. There are metered parking spaces nearby on Wright street, the Beckman circle drive and the campus parking garage east of the Beckman Institute.

Register

Registration is closed for this event.