2013 principles of fluorescence techniques

apr 8 - 11, 2013 | urbana-champaign, il

Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques workshop will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation.

The workshop is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FFS applications.

Participants are recommended to have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending. Interactions between participants and lecturers will be fostered. Students will have ample opportunity to personally explain their research programs and ask questions about the applicability of specific fluorescence techniques to their workshop lecturers. Topics addressed in this workshop include:

  • Basic Definitions and Principles of Fluorescence
  • Steady-state Fluorescence (spectra, kinetics, polarization)
  • Time-resolved Fluorescence (lifetime, anisotropy, time-resolved spectra)
  • Fluorescence Probes
  • Instrumentation
  • Data Manipulation and Data Analysis
  • Confocal and Multiphoton Fluorescence Microscopy
  • FFS, Fluorescence Fluctuation Spectroscopy
  • FLIM, Fluorescence Lifetime Imaging
  • Particle Tracking
  • Single Molecule Imaging
  • Super-resolution techniques, STED

The number of participants to the course is limited:

Lectures: Limited to a total of 70 participants (auditorium size).

Practicals: Limited to a total of 30 participants (interactive, small-group sessions).

Participation is granted on a first-registered, first-served basis.

Sponsored by:

Tentative Agenda

April 6

7:45 AM - 8:00 AM
Shuttle from Hotel to Course
8:00 AM - 8:30 AM
Breakfast
8:30 AM - 9:00 AM
Registration & Introduction
9:00 AM - 9:30 AM
A Short History of Fluorescence
Dr. Beniamino Barbieri
9:30 AM - 10:30 AM
Basic Fluorescence Principles I
Prof. David M. Jameson
10:30 AM - 10:45 AM
Coffee Break
10:45 AM - 11:45 AM
Basic Fluorescence Principles II
Prof. David M. Jameson
11:45 AM - 12:45 PM
FRET and In vitro Probes
Prof. David M. Jameson
12:45 PM - 1:15 PM
Lunch
1:15 PM - 2:15 PM
Fluorescent Probes in Cells
Prof. Richard Day
2:15 PM - 2:30 PM
Introduction to Instrumentation: Fluorometers
Prof. Dr. Martin vandeVen
2:30 PM - 2:45 PM
Introduction to Instrumentation: Microscopes
Dr. Justin Jureller
2:45 PM - 3:00 PM
Coffee Break
3:00 PM - 6:30 PM
Laboratory: Hands-on Experiments
6:30 PM - 12:00 AM
Dinner Reception
8:00 PM - 12:00 AM
Shuttle from Course to Hotel

April 7

7:45 AM - 8:00 AM
Shuttle from Hotel to Course
8:00 AM - 8:30 AM
Breakfast
8:30 AM - 9:30 AM
Fluorescence Microscopy I
Prof. Peter So
9:30 AM - 10:45 AM
Fluorescence Microscopy II
Prof. Peter So
10:45 AM - 11:00 AM
Coffee Break
11:00 AM - 12:30 PM
FCS, Autocorrelation; PCH, Cross-correlation
Prof. Enrico Gratton
12:30 PM - 1:15 PM
Lunch
1:15 PM - 2:45 PM
RICS and FLIM
Prof. Enrico Gratton
2:45 PM - 3:00 PM
Coffee Break & Group Picture
3:00 PM - 6:30 PM
Laboratory: Hands-on Experiments
7:00 PM - 12:00 AM
Shuttle from Course to Hotel

April 8

7:45 AM - 8:00 AM
Shuttle from Hotel to Course
8:00 AM - 8:30 AM
Breakfast
8:30 AM - 11:30 AM
Laboratory: Hands-on Experiments
11:30 AM - 12:30 PM
Lunch
12:30 PM - 2:30 PM
Plasmonics
Prof. Zygmunt Gryczynski
2:30 PM - 3:30 PM
Advances in Live Cell Laser Scanning Confocal and Intravital Multiphoton Imaging
Dr. James Lopez
3:30 PM - 3:45 PM
Coffee Break
3:45 PM - 4:15 PM
Closing Remarks/Award of Participation Diplomas
4:30 PM - 12:00 AM
Shuttle from Course to Hotel

Instructors

Dr. Beniamino Barbieri

Dr. Beniamino Barbieri

ISS, Inc. | President | 1602 Newton Dr. | Champaign, IL 61822 | Tel: 217-359-8681

Prof. Richard Day

Prof. Richard Day

Department of Cellular & Integrative Physiology | Indiana University School of Medicine | 635 Barnhill Drive, Room 333 | Indianapolis, IN 46202-5120 | USA

Prof. Enrico Gratton

Prof. Enrico Gratton

University of California, Irvine | Department of Biomedical Engineering | Irvine, CA 92697-2715 | USA | Tel: 949-824-2674

Prof. Zygmunt Gryczynski

Prof. Zygmunt Gryczynski

University of North Texas | Dept. of Molecular Biology and Immunology | 3500 Camp Bowie Blvd. | Fort Worth, TX 76107 | USA | Tel: 808-956-5034

Prof. David M. Jameson

Prof. David M. Jameson

University of Hawaii at Manoa | Department of Cell and Molecular Biology | John A. Burns School of Medicine | Honolulu, HI 96822 | USA | Tel: 808-956-5034 | Website

Dr. Justin Jureller

Dr. Justin Jureller

University of Chicago | Chicago, IL | USA | Tel: 773-834-3864

Dr. James Lopez

Dr. James Lopez

Olympus

Prof. Peter So

Prof. Peter So

Massachusettes Institute of Technology (MIT) | Dept. of Mechanical and Biological Engineering | Cambridge, MA 02139 | USA | Tel: 617-253-6552

Prof. Dr. Martin vandeVen

Prof. Dr. Martin vandeVen

Hasselt University and School of Life Sciences | Transnationale Universiteit Limburg | Biomedical Research Institute | Agoralaan, Bldg. C., | B-3590-Diepenbeek | Belgium | Tel: +31-(0)11-269233/martin.vandeven@uhasselt.be

Travel

Location

The Principles of Fluorescence Techniques workshop will be held at:

Beckman Institute for Advanced Science and Technology
The University of Illinois
405 North Mathews Avenue
Urbana, IL 61801

Airports

The airport code for Champaign is CMI. Other close airports are Chicago Midway (MDW), Chicago O'Hare (ORD), Indianapolis (IND) and Bloomington-Normal (BMI).
Directions from CMI
Directions from BMI
Directions from IND
Directions from ORD
Directions from MDW

Trains

Amtrak offers a line from Chicago (CHI) to Champaign (CHM).

Bus/Shuttle

There is a shuttle service from Chicago to Champaign with Peoria Charter Coach Company.

Hotel

Hampton Inn
1200 W University Ave
Urbana, IL 61801
Phone: 217-337-1100
Click on the Hampton Inn link above to reserve your room with the Fluorescence Foundation block, called Fluorescence.

Illini Union Hotel
1401 W Green St
Urbana, IL 61801
Phone: 217-333-1241

Parking

University parking passes are available for $13/day. These are good at any university metered parking space. If you would like to purchase these passes please contact the workshop coordinator for assistance, and we will have it available at registration for you. There are metered parking spaces nearby on Wright street, the Beckman circle drive and the campus parking garage east of the Beckman Institute.

Register

Registration is closed for this event.