2020 principles of fluorescence techniques

apr 7 - 10, 2020 | urbana-champaign, il

Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques workshop will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation.

The workshop is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FFS applications.

Participants are recommended to have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending. Interactions between participants and lecturers will be fostered. Students will have ample opportunity to personally explain their research programs and ask questions about the applicability of specific fluorescence techniques to their workshop lecturers. Topics addressed in this workshop include:

  • Basic Definitions and Principles of Fluorescence
  • Steady-state Fluorescence (spectra, kinetics, polarization)
  • Time-resolved Fluorescence (lifetime, anisotropy, time-resolved spectra)
  • Fluorescence Probes
  • Instrumentation
  • Data Manipulation and Data Analysis
  • Confocal and Multiphoton Fluorescence Microscopy
  • FFS, Fluorescence Fluctuation Spectroscopy
  • FLIM, Fluorescence Lifetime Imaging
  • Particle Tracking
  • Single Molecule Imaging
  • Super-resolution techniques, STED

The number of participants to the course is limited:

Lectures: Limited to a total of 70 participants (auditorium size).

Practicals: Limited to a total of 30 participants (interactive, small-group sessions).

Participation is granted on a first-registered, first-served basis.

Sponsored by:

Tentative Agenda

August 17

1:30 PM - 2:00 PM
Registration & Introductions
2:00 PM - 2:30 PM
Welcome Address
Dr. Glenn Fried
2:30 PM - 3:30 PM
Confocal Miscroscopy and Full Field Microscopy
Dr. Glenn Fried
3:30 PM - 4:30 PM
Quantitative Fluorescence Microscopy for the Study of Protein Interactions, Signaling and Molecular Microenvironment
Dr. Beniamino Barbieri
4:30 PM - 5:00 PM
Coffee Break
5:00 PM - 6:00 PM
Single Molecule Detection Instrumentation
Prof. Paul Selvin
6:00 PM - 12:00 AM
Networking Mixer

August 18

8:00 AM - 8:30 AM
Breakfast
8:30 AM - 9:30 AM
Light Sheet Microscopy
Dr. Kingsley Boateng
9:30 AM - 10:00 AM
Coffee Break
10:00 AM - 11:00 AM
Phase Imaging Microscopy
Prof. Gabriel Popescu
11:00 AM - 12:00 PM
Optical Superresolution of Cultured Neurons
Prof. Paul Selvin
12:00 PM - 1:00 PM
Lunch
1:00 PM - 1:30 PM
Group Picture and Experiment Group Assignment
1:30 PM - 2:30 PM
Superresolution and Nanoimaging
Dr. Austin J. Cyphersmith
2:30 PM - 4:25 PM
Laboratory Practicals 1
4:25 PM - 4:35 PM
Coffee Break
4:35 PM - 6:30 PM
Laboratory Practicals 2

August 19

8:00 AM - 8:30 AM
Breakfast
8:30 AM - 10:00 AM
FRET: In Vitro and In Vivo Fluorescence Probes
Prof. David M. Jameson
10:00 AM - 10:10 AM
Coffee Break
10:10 AM - 11:10 AM
Two Photon Imaging
Dr. Mayandi Sivaguru
11:10 AM - 12:00 PM
Lunch
12:00 PM - 1:00 PM
Time-resolved Fluorescence and FLIM
Prof. David M. Jameson
1:00 PM - 2:55 PM
Laboratory Practicals 3
2:55 PM - 3:05 PM
Coffee Break
3:05 PM - 5:00 PM
Laboratory Practicals 4

August 20

8:00 AM - 8:30 AM
Breakfast
8:30 AM - 10:25 AM
Laboratory Practicals 5
10:25 AM - 10:35 AM
Coffee Break
10:35 AM - 12:30 PM
Laboratory Practicals 6
12:30 PM - 1:30 PM
Lunch
1:30 PM - 2:30 PM
Label-Free Multimodal Multiphoton Microscopy
of Carcinogenesis by Shaping Fiber Supercontinuum Pulses
Prof. Stephen A. Boppart
2:30 PM - 3:30 PM
Second Harmonic Imaging of Biological Samples
Prof. Kimani Toussaint
3:30 PM - 4:30 PM
Raman Imaging of Biological Samples
Mr. Matthew Kole
4:30 PM - 5:00 PM
Coffee Break
5:00 PM - 6:00 PM
IR Imaging of Biological Samples
Dr. Tomasz Wrobel
6:00 PM - 6:30 PM
Closing Remarks/Award of Participation Diplomas

Instructors

Dr. Beniamino Barbieri

Dr. Beniamino Barbieri

ISS, Inc. | President | 1602 Newton Dr. | Champaign, IL 61822 | Tel: 217-359-8681

Dr. Kingsley Boateng

Dr. Kingsley Boateng

Research Specialist @ Microscopy Core | IGB/Room 123 | 217-300-1643

Prof. Stephen A. Boppart

Prof. Stephen A. Boppart

Beckman Institute for Advanced Science and Technology | University of Illinois at Urbana-Champaign | 405 North Mathews Avenue | Urbana, IL 61801 | Tel: (217) 333-8598

Dr. Austin J. Cyphersmith

Dr. Austin J. Cyphersmith

University of Illinois at Urbana-Champaign | Carl R. Woese Institute for Genomic Biology | 1206 W Gregory Dr | Urbana, IL 61801

Dr. Glenn Fried

Dr. Glenn Fried

Director of Core Facilities IGB | Room 15 | University of Illinois at Urbana-Champaign | 217-333-4328

Prof. David M. Jameson

Prof. David M. Jameson

University of Hawaii at Manoa | Department of Cell and Molecular Biology | John A. Burns School of Medicine | Honolulu, HI 96822 | USA | Tel: 808-956-5034 | Website

Mr. Matthew Kole

Mr. Matthew Kole

4153 Beckman Institute | 405 North Mathews Avenue | Urbana, Illinois 61801

Prof. Gabriel Popescu

Prof. Gabriel Popescu

University of Illinois at Urbana-Champaign | Assistant Professor | 4055 Beckman Institute, MC 251 | 405 N. Mathews | Urbana, Illinois 61801 | (217) 333-4840

Prof. Paul Selvin

Prof. Paul Selvin

Professor of Physics & Biophysics | Affiliate of Cell & Developmental Biology | 365 Loomis Lab of Physics | 1110 W. Green St. | University of Illinois | Urbana IL 61801 | Tel: 217-244-3371

Dr. Mayandi Sivaguru

Dr. Mayandi Sivaguru

Assistant Director of Core Facilities IGB | Room 8 | University of Illinois at Urbana-Champaign | 217-333-1214

Prof. Kimani Toussaint

Prof. Kimani Toussaint

Associate Professor | Mechanical Science and Engineering | 2119 Mechanical Engineering Lab | 105 S. Mathews | Urbana Illinois 61801 | (217) 244-4088

Dr. Tomasz Wrobel

Dr. Tomasz Wrobel

Beckman Postdoctoral Fellow

Travel

Location

The Principles of Fluorescence Techniques workshop will be held at:

Beckman Institute for Advanced Science and Technology
The University of Illinois
405 North Mathews Avenue
Urbana, IL 61801

Airports

The airport code for Champaign is CMI. Other close airports are Chicago Midway (MDW), Chicago O'Hare (ORD), Indianapolis (IND) and Bloomington-Normal (BMI).
Directions from CMI
Directions from BMI
Directions from IND
Directions from ORD
Directions from MDW

Trains

Amtrak offers a line from Chicago (CHI) to Champaign (CHM).

Bus/Shuttle

There is a shuttle service from Chicago to Champaign with Peoria Charter Coach Company.

Hotel

Hampton Inn
1200 W University Ave
Urbana, IL 61801
Phone: 217-337-1100
Click on the Hampton Inn link above to reserve your room with the Fluorescence Foundation block, called Fluorescence.

Illini Union Hotel
1401 W Green St
Urbana, IL 61801
Phone: 217-333-1241

Parking

University parking passes are available for $13/day. These are good at any university metered parking space. If you would like to purchase these passes please contact the workshop coordinator for assistance, and we will have it available at registration for you. There are metered parking spaces nearby on Wright street, the Beckman circle drive and the campus parking garage east of the Beckman Institute.

Register

Registration is closed for this event.