2023 principles of fluorescence techniques

apr 17 - 20, 2023 | urbana-champaign, il

Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques workshop will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation.

The workshop is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FFS applications.

Participants are recommended to have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending. Interactions between participants and lecturers will be fostered. Students will have ample opportunity to personally explain their research programs and ask questions about the applicability of specific fluorescence techniques to their workshop lecturers. Topics addressed in this workshop include:

  • Basic Definitions and Principles of Fluorescence
  • Steady-state Fluorescence (spectra, kinetics, polarization)
  • Time-resolved Fluorescence (lifetime, anisotropy, time-resolved spectra)
  • Fluorescence Probes
  • Instrumentation
  • Data Manipulation and Data Analysis
  • Confocal and Multiphoton Fluorescence Microscopy
  • FFS, Fluorescence Fluctuation Spectroscopy
  • FLIM, Fluorescence Lifetime Imaging
  • Particle Tracking
  • Single Molecule Imaging
  • Super-resolution techniques, STED

The number of participants to the course is limited:

Lectures: Limited to a total of 70 participants (auditorium size).

Practicals: Limited to a total of 30 participants (interactive, small-group sessions).

Participation is granted on a first-registered, first-served basis.

Tentative Agenda

January 25

12:00 AM - 12:00 AM
Instructor
Prof. Andrea C. Oliveira
12:00 AM - 12:00 AM
Invited Instructor
Prof. Luis Aguilar Cavallo
12:00 AM - 12:00 AM
Invited Instructor
Dr. Germán Günther S.
8:30 AM - 9:00 AM
Registration
9:00 AM - 9:45 AM
Basic Fluorescence Principles Including Polarization/Anisotropy
Prof. David M. Jameson
9:45 AM - 10:30 AM
Fluorescence Probes: in vitro and in vivo cells
Prof. David M. Jameson
10:30 AM - 11:00 AM
Coffee Break
11:00 AM - 11:45 AM
Microscopy Instrumentation
Prof. Andre M.O. Gomes
11:45 AM - 12:30 PM
Confocal and 2P Microscopy
Prof. Andre M.O. Gomes
12:30 PM - 2:00 PM
Lunch (not provided)
2:00 PM - 2:45 PM
FCS: Theoretical Aspects: The ABCs of FCS
Prof. Valeria Levi
2:45 PM - 3:15 PM
Coffee Break
3:15 PM - 4:00 PM
FCS: Analysis (PCH and Autocorrelation)
Prof. Enrico Gratton
4:00 PM - 4:45 PM
FCS Applications
Dr. Susana Sánchez Donoso

January 26

9:00 AM - 9:45 AM
The RICS Method
Prof. Enrico Gratton
9:45 AM - 10:30 AM
N&B
Prof. Valeria Levi
10:30 AM - 11:00 AM
Coffee Break
11:00 AM - 11:45 AM
Laurdan GP
Prof. Valeria Levi
11:45 AM - 12:30 PM
Laurdan GP Applications
Dr. Susana Sánchez Donoso
12:30 PM - 2:00 PM
Lunch (not provided)
2:00 PM - 2:45 PM
Computer Lab: FCS/RICS
2:45 PM - 3:15 PM
Coffee Break
3:15 PM - 4:00 PM
Computer Lab: FCS/RICS Continued

January 27

9:00 AM - 9:45 AM
FRET
Prof. David M. Jameson
9:45 AM - 10:30 AM
Fluorescence Lifetime: Time-domain and Frequency Domain
Prof. David M. Jameson
10:30 AM - 11:00 AM
Coffee Break
11:00 AM - 11:45 AM
The Lifetime Phasor Approach Applied to Cells
Prof. Enrico Gratton
11:45 AM - 12:30 PM
The Lifetime Phasor Approach Applied in vitro to Biomolecules
Prof. David M. Jameson
12:30 PM - 2:00 PM
Lunch (not provided)
2:00 PM - 2:45 PM
Computer Lab: RICS/N&B
2:45 PM - 3:15 PM
Coffee Break
3:15 PM - 4:45 PM
Computer Lab: RICS/N&B Continued
5:00 PM - 6:00 PM
Gregorio Weber: A Fluorescent Lifetime
Prof. David M. Jameson

January 28

9:00 AM - 9:45 AM
Particle Tracking
Prof. Valeria Levi
9:45 AM - 10:30 AM
Application Tracking
Prof. Andre M.O. Gomes
10:30 AM - 11:00 AM
Coffee Break
11:00 AM - 11:45 AM
Pair Correlation
Prof. Enrico Gratton
11:45 AM - 12:30 PM
Spectral Phasors
Prof. Enrico Gratton
12:30 PM - 2:00 PM
Lunch (not provided)
2:00 PM - 2:45 PM
Computer Lab: FLIM Phasor
2:45 PM - 3:15 PM
Coffee Break
3:15 PM - 4:00 PM
Computer Lab: FLIM Phasor Continued
4:00 PM - 12:00 AM
Closure

January 29

9:00 AM - 10:30 AM
Experimentation
10:30 AM - 11:00 AM
Coffee Break
11:00 AM - 12:30 PM
Experimentation
12:30 PM - 2:00 PM
Lunch (not provided)
2:00 PM - 2:45 PM
Experimentation
2:45 PM - 3:15 PM
Coffee Break
3:15 PM - 4:00 PM
Experimentation

Instructors

Prof. Luis Aguilar Cavallo

Prof. Luis Aguilar Cavallo

Laboratorio de Fotofísica y Espectroscopía Molecular | Instituto de Química, Facultad de Ciencias | Pontificia Universidad Católica de Valparaíso, Chile

Dr. Susana Sánchez Donoso

Dr. Susana Sánchez Donoso

Facultad de Ciencias Químicas | Departmento de Polimeros | Universidad de Concepción | Concepción, Chile

Prof. Andre M.O. Gomes

Prof. Andre M.O. Gomes

Cidade Universitária | Laboratório de Termodinâmica de Proteínas e Estruturas Virais Gregorio Weber Instituto de Bioquímica Médica | Universidade Federal do Rio de Janeiro, Brasil

Prof. Enrico Gratton

Prof. Enrico Gratton

University of California, Irvine | Department of Biomedical Engineering | Irvine, CA 92697-2715 | USA | Tel: 949-824-2674

Prof. David M. Jameson

Prof. David M. Jameson

University of Hawaii at Manoa | Department of Cell and Molecular Biology | John A. Burns School of Medicine | Honolulu, HI 96822 | USA | Tel: 808-956-5034 | Website

Prof. Valeria Levi

Prof. Valeria Levi

Laboratorio de Dinámica intracelular Facultad de Ciencias Exactas y Naturales | Universidad de Buenos Aires | Argentina

Prof. Andrea C. Oliveira

Prof. Andrea C. Oliveira

Laboratório de Termodinâmica de Proteínas e Estruturas Virais Gregorio Weber | Instituto de Bioquímica Médica | Universidade Federal do Rio de Janeiro | Brazil

Dr. Germán Günther S.

Dr. Germán Günther S.

Laboratorio de Cinética y Fotoquímica | Facultad de Ciencias Químicas y Farmacéuticas | Universidad de Chile, Santiago de Chile, Chile

Travel

Location

The Principles of Fluorescence Techniques workshop will be held at:

Beckman Institute for Advanced Science and Technology
The University of Illinois
405 North Mathews Avenue
Urbana, IL 61801

Airports

The airport code for Champaign is CMI. Other close airports are Chicago Midway (MDW), Chicago O'Hare (ORD), Indianapolis (IND) and Bloomington-Normal (BMI).
Directions from CMI
Directions from BMI
Directions from IND
Directions from ORD
Directions from MDW

Trains

Amtrak offers a line from Chicago (CHI) to Champaign (CHM).

Bus/Shuttle

There is a shuttle service from Chicago to Champaign with Peoria Charter Coach Company.

Hotel

Hampton Inn
1200 W University Ave
Urbana, IL 61801
Phone: 217-337-1100
Click on the Hampton Inn link above to reserve your room with the Fluorescence Foundation block, called Fluorescence.

Illini Union Hotel
1401 W Green St
Urbana, IL 61801
Phone: 217-333-1241

Parking

University parking passes are available for $13/day. These are good at any university metered parking space. If you would like to purchase these passes please contact the workshop coordinator for assistance, and we will have it available at registration for you. There are metered parking spaces nearby on Wright street, the Beckman circle drive and the campus parking garage east of the Beckman Institute.

Register

Registration is closed for this event.