2027 principles of fluorescence techniques

Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques workshop will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation.

The workshop is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FFS applications.

Participants are recommended to have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending. Interactions between participants and lecturers will be fostered. Students will have ample opportunity to personally explain their research programs and ask questions about the applicability of specific fluorescence techniques to their workshop lecturers. Topics addressed in this workshop include:

• Basic Definitions and Principles of Fluorescence
• Steady-state Fluorescence (spectra, kinetics, polarization)
• Time-resolved Fluorescence (lifetime, anisotropy, time-resolved spectra)
• Fluorescence Probes
• Instrumentation
• Data Manipulation and Data Analysis
• Confocal and Multiphoton Fluorescence Microscopy
• FFS, Fluorescence Fluctuation Spectroscopy
• FLIM, Fluorescence Lifetime Imaging
• Particle Tracking
• Single Molecule Imaging
• Super-resolution techniques, STED

The number of participants to the course is limited:

Lectures: Limited to a total of 70 participants (auditorium size).

Practicals: Limited to a total of 30 participants (interactive, small-group sessions).

Participation is granted on a first-registered, first-served basis.

Sponsored by:

• ISS
• Beckman Institute

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