2011 principles of fluorescence techniques

apr 6 - 8, 2011 | chicago, il

Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques workshop will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation.

The workshop is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FFS applications.

Participants are recommended to have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending. Interactions between participants and lecturers will be fostered. Students will have ample opportunity to personally explain their research programs and ask questions about the applicability of specific fluorescence techniques to their workshop lecturers. Topics addressed in this workshop include:

  • Basic Definitions and Principles of Fluorescence
  • Steady-state Fluorescence (spectra, kinetics, polarization)
  • Time-resolved Fluorescence (lifetime, anisotropy, time-resolved spectra)
  • Fluorescence Probes
  • Instrumentation
  • Data Manipulation and Data Analysis
  • Confocal and Multiphoton Fluorescence Microscopy
  • FFS, Fluorescence Fluctuation Spectroscopy
  • FLIM, Fluorescence Lifetime Imaging
  • Particle Tracking
  • Single Molecule Imaging
  • Super-resolution techniques, STED

The number of participants to the course is limited:

Lectures: Limited to a total of 70 participants (auditorium size).

Practicals: Limited to a total of 30 participants (interactive, small-group sessions).

Participation is granted on a first-registered, first-served basis.

Sponsored by:

Tentative Agenda

September 14

8:30 AM - 9:15 AM
Registration
9:30 AM - 10:00 AM
A Short History of Fluorescence - Lecture 1
Dr. Beniamino Barbieri
10:00 AM - 10:30 AM
Coffee Break
10:30 AM - 12:00 PM
Basic Fluorescence Principles I - Lecture 2
Prof. David M. Jameson
12:00 PM - 1:15 PM
Lunch
1:15 PM - 2:45 PM
Basic Fluorescence Principles II - Lecture 3
Prof. David M. Jameson
2:45 PM - 3:45 PM
Fluorescence Probes and Labels for Biomedical Applications - Lecture 4
Dr. Susana Sánchez Donoso
3:45 PM - 4:15 PM
Coffee Break
4:15 PM - 5:45 PM
Basic Instrumentation - Lecture 5
Prof. Dr. Martin vandeVen
5:45 PM - 7:45 PM
Fluorescence Microscopy I - Lecture 6
Prof. Enrico Gratton
8:00 PM - 12:00 AM
Dinner Reception

September 15

8:30 AM - 10:00 AM
FCS, Autocorrelation; PCH, Cross-correlation - Lecture 7
Prof. Enrico Gratton
10:00 AM - 10:30 AM
Coffee Break
10:30 AM - 12:00 PM
RICS and FLIM - Lecture 8
Prof. Enrico Gratton
12:00 PM - 1:30 PM
Lunch
1:30 PM - 3:00 PM
phasor-FLIM - Lecture 9
Prof. Enrico Gratton
3:00 PM - 3:45 PM
FRET - FLIM Applications - Seminar 1
Prof. Don Lamb
3:45 PM - 4:00 PM
Introduction to Instrumentation: Fluorometers - Notes 1
Prof. Dr. Martin vandeVen
4:00 PM - 4:15 PM
Introduction to Instrumentation: Groups and Hand-on Agenda - Notes 2
Dr. Valeria Caiolfa
4:15 PM - 4:45 PM
Coffee Break and Group Picture
4:45 PM - 6:45 PM
Laboratory: Hands-on Experiments - Session 1

September 16

8:30 AM - 10:30 AM
Laboratory: Hands-on Experiments - Session 2
10:30 AM - 12:30 PM
Laboratory: Hands-on Experiments - Session 3
12:30 PM - 2:00 PM
Lunch
2:00 PM - 4:00 PM
Laboratory: Hands-on Experiments - Session 4
4:00 PM - 6:00 PM
Laboratory: Hands-on Experiments - Session 5
6:00 PM - 8:00 PM
Laboratory: Hands-on Experiments - Session 6

September 17

8:30 AM - 10:30 AM
Laboratory: Hands-on Experiments - Session 7
10:30 AM - 12:30 PM
Laboratory: Hands-on Experiments - Session 8
12:30 PM - 2:00 PM
Lunch
2:00 PM - 4:00 PM
Laboratory: Hands-on Experiments - Session 9
4:00 PM - 4:30 PM
Coffee Break
4:30 PM - 5:00 PM
Short Participant Communication
Dr. Namrata Ghildiyal
5:00 PM - 5:45 PM
Application of FCS/FCCS - Seminar 2
Prof. Catherine Royer
5:45 PM - 6:30 PM
Fluorescent Molecular Tools in Medicinal Chemistry - Seminar 3
Prof. A. Ulises Acuña
6:30 PM - 6:45 PM
Closing Remarks and Award of Participation Diplomas

Instructors

Prof. A. Ulises Acuña

Prof. A. Ulises Acuña

Consejo Superior de Investigaciones Científicas | Instituto de Química Física | 119 Serrano | E-28006 Madrid | Spain | Tel: +34 (1) 561 9400

Dr. Beniamino Barbieri

Dr. Beniamino Barbieri

ISS, Inc. | President | 1602 Newton Dr. | Champaign, IL 61822 | Tel: 217-359-8681

Dr. Valeria Caiolfa

Dr. Valeria Caiolfa

CNIC | Microscopy and Dynamic Imaging | Melchor Fernández Almagro, 3 | E-28029 Madrid | Spain | Tel: +34 (91) 4531323/9; ext:2206-2207

Dr. Susana Sánchez Donoso

Dr. Susana Sánchez Donoso

Facultad de Ciencias Químicas | Departmento de Polimeros | Universidad de Concepción | Concepción, Chile

 

Dr. Namrata Ghildiyal

H.N.B. Garhwal University Srinagar India

Prof. Enrico Gratton

Prof. Enrico Gratton

University of California, Irvine | Department of Biomedical Engineering | Irvine, CA 92697-2715 | USA | Tel: 949-824-2674

Prof. David M. Jameson

Prof. David M. Jameson

University of Hawaii at Manoa | Department of Cell and Molecular Biology | John A. Burns School of Medicine | Honolulu, HI 96822 | USA | Tel: 808-956-5034 | Website

Prof. Don Lamb

Prof. Don Lamb

Ludwig-Maximilians-Universität München | Lehrstuhl für Physikalische Chemie I | Butenandtstr. 11 | D-81377 München | Germany | Tel: 089/2180-77564

Prof. Catherine Royer

Prof. Catherine Royer

CNRS | Centre de Biochimie Structurale | 29, route de Navacelles | 34090 Montpellier CEDEX | France | Tel: +33-04 67 41 79 02

Prof. Dr. Martin vandeVen

Prof. Dr. Martin vandeVen

Hasselt University and School of Life Sciences | Transnationale Universiteit Limburg | Biomedical Research Institute | Agoralaan, Bldg. C., | B-3590-Diepenbeek | Belgium | Tel: +31-(0)11-269233/martin.vandeven@uhasselt.be

Travel

The Principles of Fluorescence Techniques course will be held at:
IBD NanoBiology Facility
The University of Chicago
Gordon Center for Integrative Science
929 E 57th Street
Chicago, IL 60637
phone: (773) 834-3864
fax: (773) 834-1917

Chicago is a major airport hub easily reachable from any city within the United States. There are two major airports in Chicago: Midway and O'Hare International Airport.

Directions from Midway to Campus
Directions from O'Hare to Campus

Trains

Metra and CTA trains are available to travel from the airport to your hotel as an alternative to a taxi or bus. A CTA station is at O'Hare across terminal 2 and 3 close to the Hilton hotel.

Bus/Shuttle

There will be a shuttle from the hotel to the campus during the course.

Ramada Inn

Ramada Inn is located near Hyde Park and very close the campus.
There will also be a shuttle to transport you to and from the course location.
Accommodation fees are not included in the course fees.

4900 South Lakeshore Drive
Chicago, IL 60615
Tel: 773-288-5800
Group Code: Fluorescence Foundation

Register

Registration is closed for this event.