2008 principles of fluorescence techniques

jun 30 - jul 3, 2008 | genova, italy

Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques workshop will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation.

The workshop is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FFS applications.

Participants are recommended to have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending. Interactions between participants and lecturers will be fostered. Students will have ample opportunity to personally explain their research programs and ask questions about the applicability of specific fluorescence techniques to their workshop lecturers. Topics addressed in this workshop include:

  • Basic Definitions and Principles of Fluorescence
  • Steady-state Fluorescence (spectra, kinetics, polarization)
  • Time-resolved Fluorescence (lifetime, anisotropy, time-resolved spectra)
  • Fluorescence Probes
  • Instrumentation
  • Data Manipulation and Data Analysis
  • Confocal and Multiphoton Fluorescence Microscopy
  • FFS, Fluorescence Fluctuation Spectroscopy
  • FLIM, Fluorescence Lifetime Imaging
  • Particle Tracking
  • Single Molecule Imaging
  • Super-resolution techniques, STED

The number of participants to the course is limited:

Lectures: Limited to a total of 70 participants (auditorium size).

Practicals: Limited to a total of 30 participants (interactive, small-group sessions).

Participation is granted on a first-registered, first-served basis.

Sponsored by:

Tentative Agenda

July 4

1:30 PM - 2:00 PM
Registration & Introductions
2:00 PM - 2:30 PM
Welcome Address
Dr. Sabato D'Auria
2:30 PM - 3:30 PM
Basic Fluorescence Instrumentation
Dr. Beniamino Barbieri
3:30 PM - 4:30 PM
Basic Fluorescence Principles I:
Overview, Excitation and Emission Spectra,
Quantum Yields, Polarization
Prof. Ranieri Bizzarri
4:30 PM - 5:00 PM
Coffee Break
5:00 PM - 6:30 PM
Basic Fluorescence Principles II:
Time-Resolved Fluorescence and Quenching
Prof. Ranieri Bizzarri
6:30 PM - 12:00 AM
Networking Mixer

July 5

8:30 AM - 10:00 AM
Basic Fluorescence Principles III:
FRET: In Vitro and In Vivo Fluorescence Probes
Prof. Dr. Martin vandeVen
10:00 AM - 10:30 AM
Coffee Break
10:30 AM - 11:30 AM
Microscopy Instrumentation
Prof. Alberto Diaspro
11:30 AM - 12:30 PM
Fluorescence Applications in Biology and Medicine
Dr. Sabato D'Auria
12:30 PM - 1:30 PM
Lunch
1:30 PM - 2:00 PM
Group Picture and Experiment Group Assignment
2:00 PM - 6:00 PM
Laboratory Practicals

July 6

8:30 AM - 10:00 AM
Quest for Higher Sensitivity in Fluorescence Based Detection.
Developing Ultrasensitive Biomedical Assays
Prof. Zygmunt Gryczynski
10:00 AM - 10:30 AM
Coffee Break
10:30 AM - 12:00 PM
Studying Cellular Functions in Real-Time and Higher Resolution
Prof. Zygmunt Gryczynski
12:00 PM - 1:00 PM
Lunch
1:00 PM - 2:30 PM
Superresolution
Prof. Alberto Diaspro
2:30 PM - 6:30 PM
Laboratory Practicals
6:30 PM - 7:30 PM
Closing Remarks/Award of Participation Diplomas

Instructors

Dr. Beniamino Barbieri

Dr. Beniamino Barbieri

ISS, Inc. | President | 1602 Newton Dr. | Champaign, IL 61822 | Tel: 217-359-8681

Prof. Ranieri Bizzarri

Prof. Ranieri Bizzarri

Scuola Normale Superiore | National Enterprise for Nanoscience and Nanotechnology | Piazza dei Cavalieri, 6 | 56100 Pisa | Italy | Tel: +39-050-509-434

Dr. Sabato D'Auria

Dr. Sabato D'Auria

Director of the Institute of Food Science | CNR, Avellino

Prof. Alberto Diaspro

Prof. Alberto Diaspro

University of Genova | Department of Physics | Nanoscopy and NIC@IIT | 16146 Genova | Italy | Tel: +39-0103536426

Prof. Zygmunt Gryczynski

Prof. Zygmunt Gryczynski

University of North Texas | Dept. of Molecular Biology and Immunology | 3500 Camp Bowie Blvd. | Fort Worth, TX 76107 | USA | Tel: 808-956-5034

Prof. Dr. Martin vandeVen

Prof. Dr. Martin vandeVen

Hasselt University and School of Life Sciences | Transnationale Universiteit Limburg | Biomedical Research Institute | Agoralaan, Bldg. C., | B-3590-Diepenbeek | Belgium | Tel: +31-(0)11-269233/martin.vandeven@uhasselt.be

Travel

The Principles of Fluorescence Techniques course will be held at:
Italian Institute of Technology
via Morego, 30
1613 Genova
Phone: +39 010 71781
Fax: +39 010 720321

For a map and detailed instructions, please go to http://www.iit.it/en/location. The rest of this page provides a basic introduction to Genova.

Genova can be easily reached from major European cities.

By Plane

The Genova international airport, Cristoforo Colombo, is located 6 km from downtown Genova. It maintains direct air connections to Barcelona, Frankfurt, Geneva, London (LGW), Milan, Munich, Paris, and Zurich regularly. There are shuttle buses to Stazione Brignole, the main train station, every half hour. Buy a bus ticket on the bus for € 2.5, or buy a day ticket for € 3 (recommended), which will allow you to take any bus, including the shuttle, for the whole day. You may take a train or a taxi (approximately € 20).

By Train

Rapid train connections to the European train network occurring daily:
to/from Milan for Switzerland/Austria/Germany;
to/from Turin for North France/Paris;
to/from Menton/Marseille;
to/from Rome/Neaples and Pisa/Florence.

Rapid connections are guaranteed by the TEE, EC and IC trains with convenient times in all directions. The trains follow the routes of major European highways. The Ventimiglia-La Spezia line gathers travelers in western Italy going to Turin via Savona-Genoa, Milan via Genova, and Parma via La Spezia, and joins the Tirrenica line for Rome, Naples and the South. (Time table of Italian railways.)

By Bus

Bus tickets can be bought at newspaper kiosks or tobacco shops for € 1, or during the night you may buy them from the bus driver for € 1.50.

By Ferryboat

The Genova port is a major Mediterranean and transatlantic maritime headquarters for passengers and merchandise. Ships arrive from ports in Europe, the Far East, the Americas, Africa and other continents. Frequent connections are guaranteed on national and Mediterranean routes and for car ferries (Sardinia, Sicily, Corsica). Regular lines travel to the ports of La Spezia, Savona and Imperia. Smaller tourist ports allow recreational watercraft of every size to be docked with optimum installation and service.

By Car

Genova is connected to the main European highway network:
A7 for Milan/Switzerland/Venice/Austria/Germany;
A10 for Ventimiglia/Menton/Marseille;
A12 for Livorno/Rome/Naples.

Currency

The official currency in Italy is the Euro (€). Foreign currencies can be converted to Euros at banks, which are usually open from Monday to Friday from 8:30 to 13:30. Most hotels also exchange foreign money and travellers’ cheques.

Weather

The weather in Genova is usually mild. The temperature in June is around 64° - 75°F or 18 - 24°C.

Electricity

Electricity in Italy is supplied at 220V - 50Hz AC.

Main European towns

(Distance by highway or train) Milan, Turin (150 km), Nice (190 km), Florence (300 km), Geneva (400 km), Venice (400 km), Rome (490 km), Munich (700 km), Naples (700 km), Barcelone (900 km).

Emergency Calls

(Dialed Free of Charge)
Police emergency: 112 or 113
Medical emergency: 118

Shopping in Genova

Most shops in Genova are open between 9:00 and 19:30.

Several hotels are located in downtown Genova. We have a group reservation at Hotel SanBiagio. We will provide you the group registration code after you have registered for the course. You are responsible for making the reservation and for paying for the cost of your accommodations. This specific hotel is about 80 euros per night in a single room.

Register

Registration is closed for this event.